Title: Cytotoxicity Assessment of Heritiera
Littoralis (Aiton), Madhuca Longifolia (König) Macbr., Nerium Indicum Mill. And
Sapium Indicum (Willd.) Leaves on Artemia Salina (L.)
Authors: Nishat Fatema, Kamrul Hasan, Alimul Islam,
Nelufa Yasmin, Meherun Nesa and Nurul Islam*
Abstract
Petroleum ether, CHCl3 and CH3OH extracts of leaves of
Heritiera littoralis (Aiton), Madhuca longifolia (König) Macbr., Nerium indicum
Mill. and Sapium indicum (Willd.) were subjected to assess cytotoxicity against
Artemia salina (L.) nauplii. The petroleum ether extract of H. littoralis
leaves showed LC50 values 273.77, 97.27, 51.60, 37.12, 14.60 and 12.59 ppm
after 12, 18, 24, 30, 36 and 42 h; the CHCl3 extract showed LC50 values 733.25,
105.51, 40.72 and 18.20 ppm after 6, 12, 18 and 24 h whereas CH3OH extract
showed 73.05, 30.62, 24.56, 20.85, 16.21 and 6.71 ppm after 6, 12, 18, 24, 30
and 36 h of exposure respectively. The petroleum ether extract of M. longifolia
leaves possess LC50 values 259.35, 115.17, 56.84 and 8.73 ppm after 12, 18, 24
and 30 h; the CHCl3 extract possess LC50 values 585.43, 205.86, 112.74, 75.62,
52.84and 47.34 ppm after 12, 18, 24, 30, 36 and 42 h but CH3OH extract possess
LC50 values 185.87, 60.70, 30.11 and 15.39 ppm after 12, 18, 24 and 30 h of
exposure respectively. The petroleum ether extract of N. Indicum leaves
recorded LC50 values 249.82, 146.07, 80.23, 54.21 and 40.19 ppm after 18, 24,
30, 36 and 42 h; the CHCl3 extract gave LC50 values 36.13, 21.72, 19.03, 16.81
and 16.34 ppm after 12, 18, 24, 30 and 36 h but CH3OH extract recorded LC50
values 394.90, 129.69, 81.50, 73.10 and 37.51 ppm after 18, 24, 30, 36 and 42 h
of exposure respectively. Similarly, the petroleum ether extract of S. indicum
leaves showed LC50 values 24.79, 13.18 and 4.61 ppm after 12, 18 and 24 h; the
CHCl3 extract were 50.45, 42.64, 21.20 and 14.93 ppm after 18, 24, 30 and 36 h
of exposure and the CH3OH extract showed LC50 values 306.37, 217.18, 149.38,
73.52, 54.45 and 22.91 ppm after 12, 18, 24, 30, 36 and 42 h of exposure
respectively. The intensity of efficacy of the extracts could be arranged in
the following descending order of S. indicum (petroleum ether extract)
>H. littoralis (CH3OH extract) >M. longifolia (petroleum ether extract)
>N. indicum (CHCl3 extract).
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